Functional Characterization of Ammonium Transporter MhAMT1;2 in Malus hupehensis

The absorption and utilization of NH4+ NO3− by plant roots is closely related to soil moisture. In this study, we investigated the effect short-time drought rewatering on uptake assimilation in 1-year-old Malus hupehensis plants, as well transcription changes ammonium transporters (AMTs) nitrate (NRTs). roots, content reductase activity decreased under some extent was restored after rewatering. Expression analysis indicated that most AMTs NRTs were down-regulated while MhAMT1;2 significantly up-regulated drought-stressed roots. Therefore, function further studied through bioinformatics analysis, tissue-specific expression, subcellular localization, functional complementation uptake-defective yeast Arabidopsis mutants. Results showed mainly expressed localized cell membrane. Moreover, can mediate both mutants, transport process affected external proton concentrations ATP. present study will create a basis for exploring roles AMT members improving N use efficiency condition fruit trees.